WebThe first PCR product was diluted 10 times using Milli-Q water and used as a template for the second PCR. The second PCR was carried out with 12 cycles of a 12 μl reaction volume containing 6.0 μl 2× KAPA HiFi HotStart ReadyMix, 0.7 μl each primer (5 μM), 3.6 μl sterile distilled H 2 O and 1.0 μl template. Different combinations of ... WebHi, you can use NCBI blast, to design your specific primers, if the gene is sequenced and registered in NCBI, just you make the reference and push pick primers to pick yours, …
polymerase chain reaction / PCR Learn Science at Scitable - Nature
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comme la réaction en chaîne de la polymérase (PCR - Traduction …
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