WebJan 25, 2013 · Abstract A novel insect β-1,4-endoglucanase (DvvENGaseI) gene belonging to the glycoside hydrolasefamily (GHF) 45 was identified from the western corn rootworm, Diabrotica virgifera virgifera. The cDNA of the DvvENGaseIconsisted of a 720 bp open reading frameencoding a 239 amino-acid protein. WebMay 4, 2010 · Phylogenetic analyses suggest that insect GHF5 and GHF45 are very ancient gene families and indicate, at least in the case of GHF5, that this family likely evolved from a common ancestor rather than, as is often reported, via horizontal gene transfer. Beetle GHF45 cellulases did not cluster with other metazoan cellulases.
(PDF) Characterization of a GHF45 cellulase, AkEG21
WebN2 - A novel insect β-1,4-endoglucanase (DvvENGaseI) gene belonging to the glycoside hydrolase family (GHF) 45 was identified from the western corn rootworm, Diabrotica virgifera virgifera. The cDNA of the DvvENGaseI consisted of a 720. bp open reading frame encoding a 239 amino-acid protein. WebGHF9 cellulases with the molecular masses of 95, 65, and 45 kDa are eluted in P-1, P-3, and P-4, respectively, while GHF45 cellulase with the molecular mass of 21 kDa is eluted in P-2. Among these fractions, P-2 shows the highest activity. The 21 kDa cellulase can be purified by AKTA-FPLC (GE Healthcare) equipped by a Superdex 200 10/300 column. dragy flashing red
Molecular cloning and functional characterization of an …
WebNov 2, 2016 · The CaCel gene from Antarctic springtail Cryptopygus antarcticus codes for a cellulase belonging to the glycosyl hydrolase family 45 (GHF45). Phylogenetic, biochemical, and structural analyses revealed that the CaCel gene product (CaCel) is closely related to fungal GHF45 endo-β-1,4-glucanases ... Macromolecules WebAdditionally, carboxymethyl cellulase-producing genes obtained from insects are related to three protein families (glycosyl-hydrolase families, GHFs), namely GHF9, GHF45, and GHF5. WebThe purified 21kDa enzyme, termed AkEG21 in the present study, hydrolyzed carboxymethyl cellulose with an optimal pH and temperature at 4.5 and 40. C, … emma whisman