Pin1 activity assay kit
Web第一部分、Pin1基因敲减对SH-SY5Y神经细胞氧反常损伤的影响 目的：观察Pin1在不同时限SH-SY5Y神经细胞氧反常损伤中的表达变化，通过Pin1基因敲减，研究Pin1对细胞凋亡的影响及在线粒体凋亡通路中的作用机制，探讨Pin1在SH-SY5Y神经细胞氧反常损伤中的功能。 方法： （1）Pin1蛋白的细胞内定位 ... WebApr 8, 2024 · Liu et al. reported that an IRAK1-PIN1 axis enhances intrinsic tumor resistance to radiation therapy in p53 ... DNA damage was detected with a comet assay kit (KeyGEN, Nanjing, China) under alkaline conditions. ... s Luciferase reporter assay for detecting the activity of wild-type or mutant IRAK1 promoters in U251 cells which were transfected ...
Pin1 activity assay kit
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WebPin1 is a PPIase specific for pSer/pThr-Pro motifs; Uses recombinant human Pin 1 and substrate Succ-AEPF-pNA; Measures cis/trans isomerisation kinetics of substrate using UV/Vis spectrophotometer; Uses LiCl / Trifluoroethanol. Li+ ion holds substrate in 60% … WebHuman PIN1 ELISA Kit (HUFI02016) from Assay Genie is a high quality, simple protocol ELISA kit to measure PIN1 in serum, plasma & cell lysate samples Toggle menu Select Currency: EUR
WebSep 17, 2024 · The inhibitory activities of our Pin1 inhibitors, H-77, H-175, H-363, H-371 and H-596, have been confirmed to inhibit Pin1 enzyme activity in an in vitro PPIase assay using recombinant Pin1 ... WebApr 12, 2024 · Penicillin is the most common example of an irreversible inhibitor. It works by binding to and inhibiting the activity of transpeptidase, the enzyme responsible for forming bacterial cell walls. The absence of a cell wall allows water to get into the bacterial cell, causing it to swell, burst and die. Aspirin and nerve gas are other examples of ...
WebJun 1, 2024 · Inhibition of Pin1 isomerase activity was determined using the chymotrypsin-coupled PPIase assay, using GST-Pin1 and Suc-Ala-phos.Ser-Pro-Phe-pNA peptide substrate, as described previously 11,47. WebPin1 Activity Assays. Pin1 activity was measured as previously described , with a few modifications. Briefly, for analysis of Pin1 inhibitors, SN were pretreated for 15 min at 37°C with drugs before lysates were prepared by addition of Triton X-100 to a final concentration of 1%, followed by three freeze-thaw cycles and 20 min incubation on ice.
WebPeptidyl-prolyl isomerase (Pin1) (1) Peroxidase (3) Phosphatase (9) Phosphate (1) Plasmin (2) Plasminogen activator (2) Protease (2) Protein (1) Renin (4) Sirtuin (SIRT) (4) Sortase A (1) ... SensoLyte® 520 ADAM10 Activity Assay Kit Fluorimetric - 1 kit Cat.Number : AS-72226; Quick view SensoLyte® 520 ADAMTS13 Activity Assay Kit Fluorimetric ...
WebFeb 1, 2024 · Pin1 (protein interacting with never in mitosis A-1) is a member of the peptidyl prolyl isomerase (PPIase) family, and catalyzes cis-trans isomerization of pThr/Ser-Pro amide bonds. ... This compound inhibited Pin1 in protease-coupled assay, and formed a covalent bond with Cys113 of Pin1, as determined by ESI-MS. The acetoxymethyl ester … hereford barrs court stationWebSensoLyte® 520 HDAC Activity Assay Kit Fluorimetric. Order Now. HAT (Histone acetyltransferase) SensoLyte® HAT (p300) Assay Kit Fluorimetric. Order Now. HCV Protease. SensoLyte® 490 HCV Protease Assay Kit Fluorimetric 500 assays. Order Now. SensoLyte® 490 HCV Protease Assay Kit Fluorimetric 200 assays. hereford az post office phone numberWebThe SensoLyte® Green Pin1 Assay Kit uses fluorogenic substrate that was pretreated to convert into cis isoform. Pin1 changes substrate into trans conformation that is readily cleaved to generate fluorescent signal which can be monitored at Ex/Em=490/520nm. … matthew miller skow and nicholas stumphauzerWebJun 2, 2014 · Specific activity is > 330 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 µmole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin. Activity Assay. 1. Prepare 180ul assay buffer into a suitable container: … matthew millington asuWebThe ELISA kits listed below target PIN1, the symbol for the human gene, peptidylprolyl cis/trans isomerase, NIMA-interacting 1. The protein has an enzyme classification of Peptidylprolyl isomerase. The protein encoded by PIN1 has a predicted amino acid … hereford az zip codes listWebNote 3: The amount of Pin1 enzyme is enough for 20 assays, the amounts supplied of the rest of the components are sufficient to run 100 assays. More Pin1 enzyme can be purchased at Abcam (Cat.#51230). Note 4: Do not re-freeze and re-use Pin1 Developer … matthew miller traderWebMouse Pin1 ELISA Kit (ab209879) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Pin1 protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Pin1 with 11.2 … matthew miller snowboard racing