WebPrimeScript II RTaseは、タカラバイオが独自に開発したPrimeScript RTaseにアクセサリータンパク質を用いて更なる改良を加え、このcDNA合成の阻害要因を極限まで抑制し … WebThe ProtoScript ® II First Strand cDNA Synthesis Kit is optimized mix for higher specificity and yield of cDNA. Recombinant M-MuLV reverse transcriptase with reduced RNase H …

PrimeScript II 1st Strand cDNA Synthesis Kit - Takara

WebThe first strand cDNA synthesized with this kit can be used for variety of applications, including second strand synthesis, hybridization, PCR amplification, and real-time PCR. II. Components (for 50 reactions) PrimeScript RTase (200 U/μl) 5X PrimeScript Buffer RNase Inhibitor (40 U/μl) dNTP Mixture (10 mM each) Oligo dT Primer (50 μM ... WebThe PrimeScript 1st strand cDNA Synthesis Kit contains all of the reagents necessary for synthesis of full-length first-strand cDNA from total or poly A + RNA, including both … legendary giants pokemon shield

PrimeScript™ 1st strand cDNA Synthesis Kit by Takara Bio

WebFirst Strand cDNA Synthesis Reaction: • Denaturation of RNA and primer at 70°C for 5 minutes can remove secondary structures that may impede long cDNA synthesis. However, this step can be omitted in some cases (unpublished results). • We recommend incubation at 42°C for one hour for maximum yield and length. WebFeatures of the SuperScript™ IV First-Strand Synthesis System include: • Significantly improved resistance to a variety of inhibitors that can interfere with cDNA synthesis. • Robust and specific cDNA synthesis in a wide range of sample types. • A faster reverse transcriptase reaction that reduces incubation time from >50 min to 10 min. WebJun 15, 2024 · Total RNA from the root, stem, and leaves of A. mongholicus was reverse-transcribed into the first-strand cDNA using PrimeScript II 1st Strand cDNA Synthesis Kit (Takara, Japan). RT-qPCR analysis of gene expression was performed using three biological replicates and three technical replicates, with TB Green® Premix Ex Taq™ II (Takara) and … legendary ghost tours new orleans